Breton A M, Aigle M
Laboratoire de Biologie Cellulaire de la Levure, IBGC/CNRS UPR9026, 1 rue Camille Saint Saëns, F-33077 Bordeaux Cedex, France.
Curr Genet. 1998 Oct;34(4):280-6. doi: 10.1007/s002940050397.
The rvs mutants display phenotypes close to those described for the actin mutants: disorganization of the actin cytoskeleton, random budding of the diploids, loss of polarity and sensitivity to salt. Mutations in the RVS genes lead to synthetic lethality with a set of mutations in the actin gene, ACT1. This synthetic lethality is allele-specific regarding the act1 mutations, pointing to a region on the actin molecule where contacts with the myosin head have been described. The possible involvement of a myosin in a vital function fulfilled both by the Rvsp proteins and actin is strengthened further by the fact that the double mutants rvs167, myo1 and rvs167, myo2 are lethal and severely affected in growth respectively. These data support the idea that actin, myosin and Rvsp proteins are linked in a common functional pathway in yeast.
rvs突变体表现出与肌动蛋白突变体所描述的表型相近：肌动蛋白细胞骨架紊乱、二倍体随机出芽、极性丧失以及对盐敏感。RVS基因的突变导致与肌动蛋白基因ACT1中的一组突变产生合成致死性。这种合成致死性在act1突变方面具有等位基因特异性，指向肌动蛋白分子上一个已被描述为与肌球蛋白头部接触的区域。rvs167、myo1和rvs167、myo2双突变体分别是致死的且生长受到严重影响，这一事实进一步强化了肌球蛋白可能参与Rvsp蛋白和肌动蛋白共同完成的重要功能的观点。这些数据支持了肌动蛋白、肌球蛋白和Rvsp蛋白在酵母中通过共同功能途径相联系的观点。
