Dzugasová V, Obernauerová M, Horváthová K, Vachová M, Záková M, Subík J
Department of Microbiology and Virology, Comenius University, Mlynská dolina B2, 842 15 Bratislava, Slovak Republic.
Curr Genet. 1998 Oct;34(4):297-302. doi: 10.1007/s002940050399.
The PEL1/PGS1 gene of the yeast Saccharomyces cerevisiae is essential for the viability of rho-/rho degrees mutants and the normal cardiolipin content of cells. The PEL1-GFP fusion gene has been found to complement the pel1/pgs1 mutation and its fluorescent protein was localized to mitochondria similarly to the beta-galactosidase activity of a protein encoded by the PEL1-lacZ fusion gene. The expression of the PEL1-lacZ reporter gene was repressed in cells grown in the presence of inositol and choline, reduced in the ino2 and ino4 strains, but constitutive in the opi1 null-mutant strain. The results demonstrate that Pel1p, playing a vital role in cells impaired in the mitochondrial DNA, is localized in the mitochondria and expressed in response to inositol and choline.
酿酒酵母的PEL1/PGS1基因对于rho⁻/rho⁰突变体的生存能力以及细胞正常的心磷脂含量至关重要。已发现PEL1-GFP融合基因可弥补pel1/pgs1突变，并且其荧光蛋白定位于线粒体，这与PEL1-lacZ融合基因编码的蛋白质的β-半乳糖苷酶活性类似。PEL1-lacZ报告基因的表达在肌醇和胆碱存在下生长的细胞中受到抑制，在ino2和ino4菌株中降低，但在opi1缺失突变体菌株中组成型表达。结果表明，Pel1p在线粒体DNA受损的细胞中起重要作用，定位于线粒体并响应肌醇和胆碱而表达。
