Wilkinson J, Grimley S, Collins A, Thomas N S, Holgate S T, Morton N
University Medicine, University of Southampton, Southampton, SO16 5YA, United Kingdom.
Genomics. 1998 Nov 1;53(3):251-9. doi: 10.1006/geno.1998.5485.
We present evidence of linkage between markers on chromosome 12 and asthma using the BETA program for nonparametric single- and multipoint linkage analysis. We have used quantitative scores as our phenotypic variables, combining data into indices for asthma and atopy and maximizing heritability. The largest single-locus LODs were achieved for asthma: D12S342 and asthma score (LOD 2.255), D12S324 and asthma affection (LOD 2.214), and D12S366 and wheeze (LOD 3.307). The region of interest identified using multipoint analysis, with a maximum LOD of 2.29, centers around D12S97 at location 173.5 cM with a standard error of 6.5 for the asthma score and close agreement for asthma affection and wheeze. Such evidence merits further investigation of this area in an attempt to define the region with greater precision with a view to identifying candidate genes. We hope that the methods presented will encourage researchers to use phenotypic information in a way that encourages meta-analysis.
我们使用BETA程序进行非参数单基因和多基因连锁分析，展示了12号染色体上的标记与哮喘之间的连锁证据。我们将定量评分作为表型变量，将数据合并为哮喘和特应性指数，并最大化遗传率。哮喘获得的最大单基因位点对数优势比为：D12S342与哮喘评分（对数优势比2.255）、D12S324与哮喘患病情况（对数优势比2.214）、D12S366与喘息（对数优势比3.307）。使用多基因分析确定的感兴趣区域，最大对数优势比为2.29，以位于173.5厘摩处的D12S97为中心，哮喘评分的标准误为6.5，哮喘患病情况和喘息情况与之高度一致。这些证据值得对该区域进行进一步研究，以便更精确地界定该区域，从而识别候选基因。我们希望所介绍的方法将鼓励研究人员以促进荟萃分析的方式使用表型信息。
