Cheng L L, Chang I W, Smith B L, Gonzalez R G
Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02129, USA.
J Magn Reson. 1998 Nov;135(1):194-202. doi: 10.1006/jmre.1998.1578.
We report the results of a study of human breast ductal carcinomas, conducted by using high resolution magic angle spinning proton magnetic resonance spectroscopy (HRMAS 1HMRS). This recently developed spectroscopic technique can measure tissue metabolism from intact pathological specimens and identify tissue biochemical changes, which closely correspond to tumor in vivo state. This procedure objectively indicates diagnostic parameters, independent of the skill and experience of the investigator, and has the potential to reduce the sampling errors inherently associated with procedures of conventional histopathology. In this study, we measured 19 cases of female ductal carcinomas. Our results demonstrate that: (1) highly resolved spectra of intact specimens of human breast ductal carcinomas can be obtained; (2) carcinoma-free tissues and carcinomas are distinguishable by alterations in the intensities and the spin-spin relaxation time T2 of cellular metabolites; and (3) tumor metabolic markers, such as phosphocholine, lactate, and lipids, may correlate with the histopathological grade determined from evaluation of the adjacent specimen. Our results suggest that biochemical markers thus measured may function as a valuable adjunct to histopathology to improve the accuracy of and reduce the time frame required for the diagnosis of human breast cancer.
我们报告了一项使用高分辨率魔角旋转质子磁共振波谱（HRMAS 1HMRS）对人类乳腺导管癌进行研究的结果。这种最近开发的波谱技术能够从完整的病理标本中测量组织代谢，并识别与肿瘤体内状态密切相关的组织生化变化。该程序客观地指出诊断参数，独立于研究者的技术和经验，并且有可能减少与传统组织病理学程序固有相关的采样误差。在本研究中，我们测量了19例女性导管癌。我们的结果表明：（1）可以获得人类乳腺导管癌完整标本的高分辨率波谱；（2）无癌组织和癌组织可通过细胞代谢物强度和自旋 - 自旋弛豫时间T2的改变来区分；（3）肿瘤代谢标志物，如磷酸胆碱、乳酸和脂质，可能与根据相邻标本评估确定的组织病理学分级相关。我们的结果表明，如此测量的生化标志物可能作为组织病理学的有价值辅助手段，以提高人类乳腺癌诊断的准确性并缩短所需时间。
