Morisson M, Pitel F, Fillon V, Pouzadoux A, Bergé R, Vit J P, Zoorob R, Auffray C, Gellin J, Vignal A
Laboratoire de Génétique Cellulaire, INRA, Tolosan, France.
Anim Genet. 1998 Oct;29(5):348-55. doi: 10.1046/j.1365-2052.1998.295348.x.
As an approach to integrate the chicken genetic and cytogenetic maps, bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) clones were localized by fluorescence in situ hybridization (FISH) on chromosomes and by genetic mapping on the East Lansing and Compton reference families. Some of the clones used in this study were previously selected for the presence of potentially polymorphic (CA)n repeats and a microsatellite marker was developed when possible for genetic mapping. For other clones, a single strand conformational polymorphism (SSCP) was developed and used for this purpose. Between the two approaches, 18 markers linking the cytogenetic and genetic maps, seven on macrochromosomes and 11 on microchromosomes, were generated. Our results enabled the assignment and orientation of a linkage group to chromosome 3, together with the assignment of linkage groups to eight different microchromosomes, a fraction of the genome lacking mapping data and for which the degree of coverage by the genetic map was not well estimated previously.
作为整合鸡遗传图谱和细胞遗传图谱的一种方法，通过荧光原位杂交（FISH）将细菌人工染色体（BAC）和P1衍生人工染色体（PAC）克隆定位到染色体上，并通过在东兰辛和康普顿参考家系上进行遗传作图来定位。本研究中使用的一些克隆先前因存在潜在的多态性（CA）n重复序列而被选择，并且在可能的情况下开发了微卫星标记用于遗传作图。对于其他克隆，开发了单链构象多态性（SSCP）并用于此目的。通过这两种方法，产生了18个连接细胞遗传图谱和遗传图谱的标记，其中7个在大染色体上，11个在微染色体上。我们的结果使得一个连锁群能够定位到3号染色体上，并将连锁群定位到8条不同的微染色体上，这些微染色体是基因组中缺乏作图数据的一部分，并且之前对其遗传图谱的覆盖程度估计不足。
