Crooijmans R P, van Oers P A, Strijk J A, van der Poel J J, Groenen M A
Department of Animal Breeding, Wageningen Agricultural University, The Netherlands.
Poult Sci. 1996 Jun;75(6):746-54. doi: 10.3382/ps.0750746.
Microsatellite polymorphisms are finding increasing use in genetics. The objectives of this study were 1) to enlarge the number of markers to contribute to a well-defined linkage map of the chicken genome; and 2) to create a preliminary linkage map only based on microsatellite markers. The need for microsatellite markers is high for performing a whole genome scan for the identification of quantitative trait loci. Seventy-seven newly developed microsatellite markers that were polymorphic on either one or both of the reference populations were mapped and in combination with all previously described markers, used to construct a preliminary linkage map of the chicken genome. The 128 microsatellite markers mapped thus far cover 23 of the 38 linkage groups of the East Lansing reference population. In the case of the Compton reference population, 20 linkage groups out of 40 are covered with microsatellite markers. No linkage was found in the East Lansing population with five markers, and in the case of the Compton population four markers were unlinked. About 42 and 32% of the East Lansing and Compton maps, respectively, were covered by the 128 microsatellite markers. The microsatellite markers are well dispersed among the various linkage groups and there was no evidence for clustering of the markers within the map. With the 38 markers that were mapped on both reference populations, 10 of the East Lansing linkage groups could be associated with 13 of the Compton linkage groups.
微卫星多态性在遗传学中的应用越来越广泛。本研究的目的是：1）增加标记数量，以完善鸡基因组的连锁图谱；2）仅基于微卫星标记创建初步的连锁图谱。进行全基因组扫描以鉴定数量性状基因座时，对微卫星标记的需求很高。对77个新开发的微卫星标记进行了定位，这些标记在一个或两个参考群体中具有多态性，并与所有先前描述的标记相结合，用于构建鸡基因组的初步连锁图谱。到目前为止，已定位的128个微卫星标记覆盖了东兰辛参考群体38个连锁群中的23个。在康普顿参考群体中，40个连锁群中有20个被微卫星标记覆盖。在东兰辛群体中，有5个标记未发现连锁关系；在康普顿群体中，有4个标记未连锁。128个微卫星标记分别覆盖了东兰辛图谱和康普顿图谱的约42%和32%。微卫星标记在各个连锁群中分布良好，图谱中没有标记聚集的证据。利用在两个参考群体中都定位的38个标记，东兰辛的10个连锁群可与康普顿的13个连锁群相关联。
