Li S, Zadworny D, Aggrey S E, Kuhnlein U
Department of Animal Science, McGill University, St Anne de Bellevue, Canada.
Anim Genet. 1998 Oct;29(5):395-7. doi: 10.1046/j.1365-2052.1998.295362.x.
The gene coding for the mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M), a pivotal component in gluconeogenesis from lactate via the Cori cycle, was highly polymorphic in strains of egg-type chickens (White Leghorn) of different origins. Based on MspI restriction fragment polymorphisms a total of seven alleles could be distinguished. The allele frequencies were determined in six pairs of strains derived from different genetic base populations. Each pair consisted of two strains which differed in their susceptibility to Marek's disease (MD), a virus-induced neoplastic disease. The frequency of the most common haplotype (M2) was consistently higher in the susceptible strains than in the corresponding resistant strains (P < 0.05, Wilcoxon signed-ranks test), indicating that the observed differences were not due to random genetic drift. This result suggests that PEPCK-M may be a candidate gene which contains genetic variants affecting MD susceptibility. Variations in gluconeogenesis may affect the interplay between proliferation of neoplasia and host metabolism.
编码线粒体磷酸烯醇式丙酮酸羧激酶（PEPCK-M）的基因是通过科里循环由乳酸进行糖异生的关键组成部分，在不同来源的蛋鸡品种（白来航鸡）品系中具有高度多态性。基于MspI限制性片段多态性，总共可区分出七个等位基因。在源自不同遗传基础群体的六对品系中测定了等位基因频率。每对由两个品系组成，它们对马立克氏病（MD）（一种病毒诱导的肿瘤性疾病）的易感性不同。在易感品系中，最常见单倍型（M2）的频率始终高于相应的抗性品系（P < 0.05，威尔科克森符号秩检验），表明观察到的差异并非由于随机遗传漂变。这一结果表明，PEPCK-M可能是一个包含影响MD易感性的遗传变异的候选基因。糖异生的变化可能会影响肿瘤增殖与宿主代谢之间的相互作用。
