Lakshmanan N, Gavora J S, Lamont S J
Department of Animal Science, Iowa State University, Ames 50011-3150, USA.
Poult Sci. 1997 Nov;76(11):1517-23. doi: 10.1093/ps/76.11.1517.
The objective of this study was to investigate frequencies of major histocompatibility complex (MHC) class II restriction fragments in two groups of White Leghorn strains. Each group consisted of an unselected control, a strain selected for egg production traits, and a strain selected for egg production traits and Marek's disease (MD) resistance. PvuII-digested genomic DNA was hybridized with a chicken genomic MHC class II probe. The MHC class II DNA fragment frequencies in the selected strains differed from those in the related unselected control and in the strain selected using the same criteria from a different base population. Based on the sizes of the breeding populations, particularly those in the control strain and in the strain selected for egg production, it was considered unlikely that the observed changes of the MHC class II fragment frequencies were due to random genetic drift. The data suggested that some MHC class II bands are associated with production traits or with MD resistance, and that these associations tend to be unique to each genetic background. Hence, MHC class II genes are likely candidates for the investigation of quantitative trait loci in egg production and disease resistance traits such as those for which the studied strains were selected.
本研究的目的是调查两组白来航鸡品系中主要组织相容性复合体（MHC）II类限制性片段的频率。每组包括一个未经选择的对照组、一个根据产蛋性状选择的品系，以及一个根据产蛋性状和马立克氏病（MD）抗性选择的品系。用PvuII消化的基因组DNA与鸡基因组MHC II类探针杂交。所选品系中的MHC II类DNA片段频率与相关的未经选择的对照组以及从不同基础群体中使用相同标准选择的品系中的频率不同。基于育种群体的规模，特别是对照组和产蛋性状选择品系中的群体规模，认为观察到的MHC II类片段频率变化不太可能是由于随机遗传漂变所致。数据表明，一些MHC II类条带与生产性状或MD抗性相关，并且这些关联在每个遗传背景下往往是独特的。因此，MHC II类基因可能是用于研究产蛋和抗病性状（如所研究品系所选择的性状）数量性状位点的合适候选基因。
