Keserü G M
Technical University of Budapest.
Sci Prog. 1998;81 ( Pt 3):245-72.
Increased rate of cytochrome P450 catalysed metabolism is a characteristic factor in insecticide resistance. In addition to in vivo and in vitro methods, models of structural and theoretical biochemistry as well as bioorganic studies were found to be useful for understanding this increased metabolic activity on a molecular basis. Metabolic profiles can be reproduced using P450 mimic synthetic porphyrins and predicted by computer assisted biomolecular modelling. Insight into the mechanism of metabolic reactions might lead to specific P450 inhibitors reducing metabolic resistance and the environmental risk of high doses of pesticides.
细胞色素P450催化代谢速率的提高是杀虫剂抗性的一个特征因素。除了体内和体外方法外，结构和理论生物化学模型以及生物有机研究被发现有助于从分子层面理解这种增加的代谢活性。代谢谱可以使用P450模拟合成卟啉来重现，并通过计算机辅助生物分子建模进行预测。深入了解代谢反应机制可能会产生特异性P450抑制剂，从而降低代谢抗性以及高剂量农药的环境风险。
