Farthing D, Fakhry I, Ripley E B, Sica D
Division of Clinical Pharmacology and Hypertension, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0160, USA.
J Pharm Biomed Anal. 1998 Sep;17(8):1455-9. doi: 10.1016/s0731-7085(98)00021-1.
A simple high performance liquid chromatographic (HPLC) method utilizing narrowbore chromatography was developed for the determination of hydrochlorothiazide in human urine. A mobile phase of 0.1% aqueous acetic acid--acetonitrile (93:7, v/v) pH 3 was used with a C18 analytical column and ultraviolet detection (UV). The method demonstrated linearity from 2 to 50 micrograms ml-1 using 50 microliters of urine with a detection limit of 1 microgram ml-1. The method was utilized in a study evaluating if racial differences are present in the pharmacokinetic and pharmacodynamic effects of hydrochlorothiazide.
开发了一种利用窄孔色谱的简单高效液相色谱（HPLC）方法，用于测定人尿中的氢氯噻嗪。流动相为0.1%的乙酸水溶液-乙腈（93:7，v/v），pH值为3，使用C18分析柱和紫外检测（UV）。该方法使用50微升尿液，在2至50微克/毫升范围内呈线性，检测限为1微克/毫升。该方法用于一项评估氢氯噻嗪的药代动力学和药效学效应是否存在种族差异的研究。
