Peshenko I V, Novoselov V I, Evdokimov V A, Nikolaev Y V, Kamzalov S S, Shuvaeva T M, Lipkin V M, Fesenko E E
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region.
Free Radic Biol Med. 1998 Oct;25(6):654-9. doi: 10.1016/s0891-5849(98)00111-7.
The 28 kDa secretory protein is one of the abundant water-soluble proteins in olfactory epithelium of mammals. Analysis of partial amino acid sequence of the 28 kDa protein strongly suggested that it belongs to a new family of highly conserved antioxidant proteins requiring thiol for their antioxidant activity (TSA/AhpC family). In the present study, we found the 28 kDa protein to have thiol-dependent antioxidant activity, thereby protecting radical-sensitive proteins such as glutamine synthetase and hemoglobin from oxidative modification caused by thiol-dependent metal ion-catalyzed oxidation system. The purified 28 kDa protein did not possess catalase or glutathione peroxidase activities, and required thiols to exhibit its antioxidant activity. The 28 kDa protein is the first member of the family of thiol-specific antioxidants identified in olfactory epithelium and the first secretory protein shown to be thiol-specific antioxidant.
28 kDa分泌蛋白是哺乳动物嗅觉上皮中含量丰富的水溶性蛋白之一。对该28 kDa蛋白部分氨基酸序列的分析强烈表明，它属于一个高度保守的抗氧化蛋白新家族，其抗氧化活性需要硫醇（TSA/AhpC家族）。在本研究中，我们发现该28 kDa蛋白具有硫醇依赖性抗氧化活性，从而保护诸如谷氨酰胺合成酶和血红蛋白等对自由基敏感的蛋白免受硫醇依赖性金属离子催化氧化系统引起的氧化修饰。纯化的28 kDa蛋白不具备过氧化氢酶或谷胱甘肽过氧化物酶活性，并且需要硫醇来展现其抗氧化活性。该28 kDa蛋白是在嗅觉上皮中鉴定出的硫醇特异性抗氧化剂家族的首个成员，也是首个被证明为硫醇特异性抗氧化剂的分泌蛋白。
