Michaels E K, Ghosh L, Nakagawa Y, Netzer M F, Vidal P, Arsenault D, Ito H
Department of Urology, University of Illinois College of Medicine at Chicago, 60612-7316, USA.
Urology. 1998 Nov;52(5):920-4. doi: 10.1016/s0090-4295(98)00387-2.
Nephrocalcin (NC), an acidic glycoprotein produced by renal proximal tubule cells and functioning as an inhibitor of calcium oxalate monohydrate crystalization, has been previously shown to have increased urinary excretion in patients with renal cell carcinoma (RCC). The current study uses immunohistochemical techniques to localize NC to cells of primary RCC.
We studied 29 kidneys removed because of RCC. Slides were deparaffinized and stained after incubating with anti-NC antibody by using the avidin-biotin-peroxidase complex techniques. Uptake of stain by tumor cells and adjacent normal renal cells was compared.
Twenty-seven kidneys (93%) showed positive staining for RCC tumor cells; 2 kidneys staining positive for normal proximal tubule cells failed to stain adjacent RCC tumor cells (7%).
The data suggest that enhanced production of urinary NC in patients with RCC derives from cells of the primary tumor.
肾钙素（NC）是一种由肾近端小管细胞产生的酸性糖蛋白，具有抑制一水草酸钙结晶的作用，此前已有研究表明，肾细胞癌（RCC）患者尿中NC排泄增加。本研究采用免疫组化技术将NC定位到原发性RCC细胞中。
我们研究了29个因RCC而切除的肾脏。切片经脱蜡处理后，通过抗生物素蛋白-生物素-过氧化物酶复合物技术与抗NC抗体孵育后进行染色。比较肿瘤细胞和相邻正常肾细胞对染色的摄取情况。
27个肾脏（93%）的RCC肿瘤细胞呈阳性染色；2个正常近端小管细胞染色阳性的肾脏，其相邻的RCC肿瘤细胞未染色（7%）。
数据表明，RCC患者尿中NC的产生增加源于原发性肿瘤细胞。
