Ball S, Reeve M A, Robinson P S, Hill F, Brown D M, Loakes D
Nycomed Amersham plc, Amersham Laboratories, White Lion Road, Amersham, Buckinghamshire HP7 9LL, UK.
Nucleic Acids Res. 1998 Nov 15;26(22):5225-7. doi: 10.1093/nar/26.22.5225.
Studies have been carried out on the use of octamer oligonucleotides tailed with different base analogues as primers in cycle sequencing reactions. 5-Nitroindole tails improved the performance as primers of a number of octamers. A tail length of three or four 5-nitroindole residues significantly increased the sequencing signal intensity for almost all primers. The use of incomplete libraries of tailed octamer primers for primer walking is discussed.
已开展了关于使用带有不同碱基类似物尾巴的八聚体寡核苷酸作为循环测序反应引物的研究。5-硝基吲哚尾巴提高了多种八聚体作为引物的性能。三或四个5-硝基吲哚残基的尾巴长度几乎显著增加了所有引物的测序信号强度。本文讨论了使用带有尾巴的八聚体引物的不完整文库进行引物步移的情况。
