Schmitz G, Dotzauer A
Department of Virology, University of Bremen, Leobener Strasse/UFT, D-28359 Bremen, Germany.
Nucleic Acids Res. 1998 Nov 15;26(22):5230-2. doi: 10.1093/nar/26.22.5230.
The detection of hepatitis A virus (HAV) negative-strand RNA, which is synthesized during replication of the positive-strand RNA genome, proved to be difficult. We developed a method for the specific detection of HAV negative-strand RNA by RNA-DNA hybridization and luminescence detection using an anti-RNA:DNA hybrid antibody. This method, which is also applicable for the specific detection of positive-strand RNA, offers a simple, yet relatively rapid and certain means of detecting low amounts of RNA such as HAV negative-strand RNA. By using appropriate hybridization DNA probes, the method should be applicable for the detection of single-stranded RNA species of different viruses in general.
在正链RNA基因组复制过程中合成的甲型肝炎病毒（HAV）负链RNA的检测被证明是困难的。我们开发了一种通过RNA-DNA杂交和使用抗RNA:DNA杂交抗体的发光检测来特异性检测HAV负链RNA的方法。这种方法也适用于正链RNA的特异性检测，为检测低量RNA（如HAV负链RNA）提供了一种简单、相对快速且可靠的手段。通过使用合适的杂交DNA探针，该方法通常应适用于检测不同病毒的单链RNA种类。
