Szántó Z, Papp L
Lajos Kossuth University, Dept. of Inorganic and Analytical Chemistry, P.O. Box 21, Egyetem tér 1, 4032 Debrecen, Hungary.
J Control Release. 1998 Dec 4;56(1-3):239-47. doi: 10.1016/s0168-3659(98)00092-3.
The calcium content of mud patches used for therapy is very small. Several mineral clays originating from Hungary served as a base material for experiments in order to find a suitable drug for transdermal introduction of calcium ions into the body. The Ca++ transport through the pig skin has been investigated in vitro in diffusion cells applying iontophoresis. Studies of electrical and physicochemical factors acting on the permeation kinetics of in vitro experiments were performed. The utilization of direct current has intensified the Ca++ transport through the pig skin (129.78+/-26. 15 microgram Ca/cm2). On using pulsate currents the amount of the Ca++ penetrating through the skin was 5-10 times higher (283.18+/-16.89 microgram Ca/cm2, 388.71+/-19.90 microgram Ca/cm2) than that of the passive transport (36.22+/-14.20 microgram Ca/cm2). The amount of Ca++ cumulated in the receptor compartment was directly proportional to the amount of bentonite (a natural mineral clay with a large cation exchange capacity) in the donor compartment and to the concentration of Ca++ in the lattice of the applied mineral clay. Therefore, the experiments were carried out on a bentonite previously enriched in Ca++ in its lattice (50 mg Ca/g bentonite). The results of the in vitro studies could open a new field of application in the therapy of osteoporosis or in the use of mineral substances.
用于治疗的泥膏中的钙含量非常少。源自匈牙利的几种矿物黏土被用作实验的基础材料，以便找到一种将钙离子经皮导入体内的合适药物。在体外扩散池中采用离子电渗疗法研究了钙离子通过猪皮的转运情况。对体外实验中影响渗透动力学的电学和物理化学因素进行了研究。直流电的使用增强了钙离子通过猪皮的转运（129.78±26.15微克钙/平方厘米）。使用脉动电流时，透过皮肤的钙离子量比被动转运时高5至10倍（283.18±16.89微克钙/平方厘米，388.71±19.90微克钙/平方厘米），而被动转运时为（36.22±14.20微克钙/平方厘米）。受体隔室中累积的钙离子量与供体隔室中膨润土（一种具有大阳离子交换容量的天然矿物黏土）的量以及所用矿物黏土晶格中钙离子的浓度成正比。因此，实验是在晶格中预先富集钙离子（50毫克钙/克膨润土）的膨润土上进行的。体外研究结果可能会在骨质疏松症治疗或矿物质使用方面开辟一个新的应用领域。
