对染色体和上游结合因子免疫标记的核仁组织区的三维研究表明，它们在有丝分裂期间呈非随机空间排列。
The three-dimensional study of chromosomes and upstream binding factor-immunolabeled nucleolar organizer regions demonstrates their nonrandom spatial arrangement during mitosis.
作者信息
Klein C, Cheutin T, O'Donohue M F, Rothblum L, Kaplan H, Beorchia A, Lucas L, Héliot L, Ploton D
机构信息
Unité 314 Institut National de la Santé et de la Recherche Médicale, Laboratoire Pol Bouin and IFR 53, 51092 Reims Cedex France.
出版信息
Mol Biol Cell. 1998 Nov;9(11):3147-59. doi: 10.1091/mbc.9.11.3147.
Abstract
The volumic rearrangement of both chromosomes and immunolabeled upstream binding factor in entire well-preserved mitotic cells was studied by confocal microscopy. By using high-quality three-dimensional visualization and tomography, it was possible to investigate interactively the volumic organization of chromosome sets and to focus on their internal characteristics. More particularly, this study demonstrates the nonrandom positioning of metaphase chromosomes bearing nucleolar organizer regions as revealed by their positive upstream binding factor immunolabeling. During the complex morphogenesis of the progeny nuclei from anaphase to late telophase, the equal partitioning of the nucleolar organizer regions is demonstrated by quantification, and their typical nonrandom central positioning within the chromosome sets is revealed.
摘要
通过共聚焦显微镜研究了完整保存的有丝分裂细胞中染色体和免疫标记的上游结合因子的体积重排。利用高质量的三维可视化和断层扫描技术，可以交互式地研究染色体组的体积组织，并关注其内部特征。更具体地说，这项研究表明，带有核仁组织区的中期染色体通过其上游结合因子免疫标记呈阳性，显示出非随机定位。在从后期到末期的子代细胞核复杂形态发生过程中，通过定量分析证明了核仁组织区的均等分配，并揭示了它们在染色体组内典型的非随机中心定位。
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