Kozáková H, Stĕpánková R, Reháková Z, Kolínská J
Department of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Physiol Res. 1998;47(4):253-8.
The aim of this study was to evaluate the levels of disaccharidase and dipeptidyl peptidase i.v. activities in rat jejunal enterocytes under the influence of long-term germ-free conditions. We found that the brush-border lactase and dipeptidyl peptidase i.v. activities were two to three times higher in 2-month-old germ-free rats in comparison with their conventional counterparts. The highest effect of germ-free condition was observed on lactase activity in 6-month-old and dipeptidyl peptidase i.v. in 2-month-old rats. No difference between germ-free and conventional rats in sucrase and glucoamylase activities was found in 2-month-old rats. The difference develops with increasing age, sucrase activity becoming significantly higher in 6- and 12-month-old rats and glucoamylase in 12-month-old germ-free rats.
本研究的目的是评估长期无菌条件影响下大鼠空肠肠上皮细胞中二糖酶和二肽基肽酶IV的活性水平。我们发现，与普通大鼠相比，2月龄无菌大鼠的刷状缘乳糖酶和二肽基肽酶IV活性高出两到三倍。在6月龄大鼠的乳糖酶活性以及2月龄大鼠的二肽基肽酶IV活性方面，观察到无菌条件的影响最为显著。在2月龄大鼠中，无菌大鼠和普通大鼠的蔗糖酶和葡糖淀粉酶活性没有差异。随着年龄增长，差异逐渐显现，6月龄和12月龄大鼠的蔗糖酶活性显著升高，12月龄无菌大鼠的葡糖淀粉酶活性显著升高。
