重组人胰岛素样生长因子-I对链脲佐菌素诱导糖尿病大鼠葡萄糖转运蛋白GLUT 2和GLUT 4表达的影响

Effect of recombinant human insulin-like growth factor-I on expression of glucose transporters, GLUT 2 and GLUT 4, in streptozotocin-diabetic rat.

作者信息

Asada T, Ogawa T, Iwai M, Kobayashi M

机构信息

Molecular Biological Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.

出版信息

Jpn J Pharmacol. 1998 Sep;78(1):63-7. doi: 10.1254/jjp.78.63.

DOI:10.1254/jjp.78.63

PMID:9804063

Abstract

We investigated the effect of recombinant insulin-like growth factor-I (rhIGF-I) on the expression of glucose transporters, GLUT 2 in the liver and GLUT 4 in the heart, in streptozotocin (STZ)-diabetic rats by the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. GLUT 2 and its mRNA in the liver was elevated, whereas GLUT 4 and its mRNA in the heart were decreased in STZ-diabetic rats. A two-week treatment with rhIGF-I mostly restored the expression of GLUT 2 and GLUT 4 to normal rat level. We demonstrated that the effect of IGF-I on the expressions of GLUTs was almost the same as that of insulin.

摘要

我们通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，研究了重组胰岛素样生长因子-I（rhIGF-I）对链脲佐菌素（STZ）诱导的糖尿病大鼠肝脏中葡萄糖转运蛋白GLUT 2以及心脏中GLUT 4表达的影响。在STZ诱导的糖尿病大鼠中，肝脏中的GLUT 2及其mRNA水平升高，而心脏中的GLUT 4及其mRNA水平降低。用rhIGF-I进行为期两周的治疗，可使GLUT 2和GLUT 4的表达大部分恢复到正常大鼠水平。我们证明，IGF-I对葡萄糖转运蛋白表达的影响与胰岛素的影响几乎相同。