Suzuki S, Tanaka S, Nemoto K, Ichikawa A
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
FEBS Lett. 1998 Oct 16;437(1-2):44-8. doi: 10.1016/s0014-5793(98)01195-8.
The role of the C-terminal region of the 74-kDa form of L-histidine decarboxylase (HDC) in the targeting to the endoplasmic reticulum (ER) was investigated in COS-7 cells. The deletion of a 10-kDa segment (residues 578-662) of the C-terminal end of HDC, especially a 20 amino acid sequence (residues 588-607), abrogated the targeting to the ER. The C-terminal 10-kDa portion is sufficient to target the green fluorescent protein (GFP) to the ER. The 74-kDa form of HDC synthesized in an in vitro translation system post-translationally associated with the heterogeneous canine microsomal membranes. These results suggest that the C-terminal 10-kDa portion of HDC contains a signal necessary for HDC to be targeted to the ER membrane.
在COS-7细胞中研究了L-组氨酸脱羧酶（HDC）74-kDa形式的C末端区域在靶向内质网（ER）中的作用。删除HDC C末端的一个10-kDa片段（第578-662位氨基酸），尤其是一个20个氨基酸的序列（第588-607位氨基酸），会消除其向内质网的靶向作用。C末端的10-kDa部分足以将绿色荧光蛋白（GFP）靶向内质网。在体外翻译系统中合成的74-kDa形式的HDC在翻译后与异源犬微粒体膜结合。这些结果表明，HDC的C末端10-kDa部分包含HDC靶向内质网的必要信号。
