Woo E J, Dunwell J M, Goodenough P W, Pickersgill R W
School of Plant Sciences, The University of Reading, Whiteknights, UK.
FEBS Lett. 1998 Oct 16;437(1-2):87-90. doi: 10.1016/s0014-5793(98)01203-4.
The oxalate oxidase enzyme expressed in barley roots is a thermostable, protease-resistant enzyme that generates H2O2. It has great medical importance because of its use to assay plasma and urinary oxalate, and it has also been used to generate transgenic, pathogen-resistant crops. This protein has now been purified and three types of crystals grown. X-ray analysis shows that the symmetry present in these crystals is consistent with a hexameric arrangement of subunits, probably a trimer of dimers. This structure may be similar to that found in the related seed storage proteins.
大麦根中表达的草酸氧化酶是一种耐热、抗蛋白酶的酶，可产生过氧化氢。由于其用于检测血浆和尿液中的草酸，因此具有重要的医学意义，并且还被用于培育转基因抗病作物。这种蛋白质现已被纯化，并培养出了三种类型的晶体。X射线分析表明，这些晶体中的对称性与亚基的六聚体排列一致，可能是二聚体的三聚体。这种结构可能与相关种子储存蛋白中的结构相似。
