Koong M K, Jun J H, Song S J, Lee H J, Song I O, Kang I S
Department of Obstetrics and Gynecology, Samsung Cheil Hospital and Women's Healthcare Center, College of Medicine, Sungkyunkwan University, Seoul, Korea.
Hum Reprod. 1998 Oct;13(1O):2852-6. doi: 10.1093/humrep/13.10.2852.
The purpose of this study was to evaluate whether hydrosalpingeal fluid (HSF) is toxic to the mouse embryo as assessed by the blastocyst development rate (BDR) and by cell counting in vitro. HSF was collected from nine patients undergoing salpingoneostomy to correct hydrosalpinx. Two-cell embryos were obtained from superovulated ICR mice. T6 medium and T6 + 0.4% bovine serum albumin (BSA) were used as control media. T6 medium containing 10% or 50% HSF and 100% HSF from each patient were used as test media. Nine to 15 embryos were cultured in microdrops prepared from each of these media. The BDR was examined after 72 h of culture in these media. To assess the total cell number within each blastocyst, the blastocysts were fixed and stained with Hoechst 33342 to facilitate cell counting. The BDR was affected adversely only by 100% HSF and not in media containing 10% or 50% HSF. The mean BDR using T6 medium and T6 + BSA were 88.7% and 85.3%, respectively. The mean BDR using media containing 10% HSF or 50% HSF were 90.0% and 89.4%, respectively. Mean BDR using 100% HSF was 75.2% (P < 0.05). The overall mean cell counts (+/- SEM) using T6 medium and T6 + BSA were 86.9+/-3.2 and 91.0+/-3.8 respectively. Mean cells counts were decreased significantly only in blastocysts cultured in 100% HSF (63.3+/-4.6; P < 0.01) but not in blastocysts cultured in 10% or 50% HSF (90.8+/-4.2 and 81.9+/-6.1 respectively). Thus, it is concluded that HSF has no embryotoxic effect but has a mildly negative effect on embryonic growth and development.
本研究的目的是通过囊胚发育率（BDR）和体外细胞计数来评估输卵管积水液（HSF）对小鼠胚胎是否有毒性。从9名接受输卵管造口术以矫正输卵管积水的患者中收集HSF。从超排卵的ICR小鼠中获取二细胞胚胎。使用T6培养基和T6 + 0.4%牛血清白蛋白（BSA）作为对照培养基。将含有10%或50% HSF以及来自每位患者的100% HSF的T6培养基用作试验培养基。在由这些培养基制备的微滴中培养9至15个胚胎。在这些培养基中培养72小时后检查BDR。为了评估每个囊胚内的总细胞数，将囊胚固定并用Hoechst 33342染色以方便细胞计数。仅100% HSF对BDR有不利影响，而含10%或50% HSF的培养基则无此影响。使用T6培养基和T6 + BSA的平均BDR分别为88.7%和85.3%。使用含10% HSF或50% HSF培养基的平均BDR分别为90.0%和89.4%。使用100% HSF的平均BDR为75.2%（P < 0.05）。使用T6培养基和T6 + BSA的总体平均细胞计数（±SEM）分别为86.9±3.2和91.0±3.8。仅在100% HSF中培养的囊胚的平均细胞数显著减少（63.3±4.6；P < 0.01），而在10%或50% HSF中培养的囊胚则未减少（分别为90.8±4.2和81.9±6.1）。因此，得出结论：HSF没有胚胎毒性作用，但对胚胎生长发育有轻度负面影响。
