Jones A M, Im K H, Savka M A, Wu M J, DeWitt N G, Shillito R, Binns A N
Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA.
Science. 1998 Nov 6;282(5391):1114-7. doi: 10.1126/science.282.5391.1114.
To test the hypothesis that auxin-binding protein 1 (ABP1) is a receptor controlling auxin-mediated plant cell expansion, ABP1 complementary DNAs were expressed in a controllable fashion in tobacco plants and constitutively in maize cell lines. Induction of Arabidopsis ABP1 expression in tobacco leaf strips resulted in an increased capacity for auxin-mediated cell expansion, whereas induction of ABP1 in intact plants resulted in leaves with a normal morphology, but larger cells. Similarly, constitutive expression of maize ABP1 in maize cell lines conferred on them the capacity to respond to auxin by increasing cell size. These results support a role of ABP1 as an auxin receptor controlling plant growth.
为了验证生长素结合蛋白1（ABP1）是控制生长素介导的植物细胞扩张的受体这一假说，ABP1互补DNA在烟草植株中以可控方式表达，并在玉米细胞系中组成型表达。在烟草叶条中诱导拟南芥ABP1表达导致生长素介导的细胞扩张能力增强，而在完整植株中诱导ABP1表达则导致叶片形态正常但细胞更大。同样，玉米ABP1在玉米细胞系中的组成型表达赋予它们通过增大细胞大小来响应生长素的能力。这些结果支持ABP1作为控制植物生长的生长素受体的作用。
