Zhang D, Dhillon H, Prasad M R, Markesbery W R
Department of Surgery, University of Kentucky Chandler Medical Center, Lexington, KY, USA.
Brain Res. 1998 Nov 16;811(1-2):161-5. doi: 10.1016/s0006-8993(98)00935-4.
The levels of PLCgamma, a phospholipase C (PLC) isozyme, were higher in the cytosol fraction than in the membrane fraction in several control brain regions. The levels of PLCgamma were significantly elevated in the membrane, but not in the cytosolic fraction of the hippocampus of AD subjects. In the superior and middle temporal gyri (SMTG) of AD subjects, the levels of PLCgamma were significantly elevated in both the membrane and cytosolic fractions. In the inferior parietal lobule and cerebellum of AD subjects, no significant changes were found in the PLCgamma levels of either cytosolic or membrane fractions. These results suggest that the increased levels of PLCgamma, by increasing the hydrolysis of PIP2 in the hippocampus and SMTG, may contribute to pathophysiology of AD. These results also support a role for excitatory neurotransmitters and their receptors in AD.
磷脂酶C（PLC）同工酶PLCγ的水平在几个对照脑区的胞质组分中高于膜组分。在阿尔茨海默病（AD）患者海马体的膜组分中，PLCγ水平显著升高，但在胞质组分中未升高。在AD患者的颞上回和颞中回（SMTG），膜组分和胞质组分中的PLCγ水平均显著升高。在AD患者的顶下小叶和小脑中，胞质或膜组分中的PLCγ水平均未发现显著变化。这些结果表明，PLCγ水平的升高通过增加海马体和SMTG中磷脂酰肌醇-4,5-二磷酸（PIP2）的水解，可能有助于AD的病理生理过程。这些结果也支持兴奋性神经递质及其受体在AD中的作用。
