Baqui A A, Meiller T F, Turng B F, Kelley J I, Falkler W A
Department of Oral Medicine, Dental School, University of Maryland, Baltimore 21201, USA.
Immunopharmacol Immunotoxicol. 1998 Nov;20(4):493-518. doi: 10.3109/08923979809031512.
A human THP-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (GM-CSF) supplementation with lipopolysaccharide (LPS) of oral microorganisms to stimulate monocyte/macrophage functional activity. LPS of oral microorganisms, Fusobacterium nucleatum and Porphyromonas gingivalis was produced by phenol-water extraction and characterized. The phagocytosis assay was performed using F1TC labeled Saccharomyces yeast particles. Phagocytic functional activity was observed in 10-11% of resting THP-1 cells. Treatment of THP-1 cells with LPS of F. nucleatum or P. gingivalis increased the phagocytic activity of THP-1 cells 2-3 fold. GM-CSF significantly increased phagocytosis either alone or when supplemented with LPS of F. nucleatum or P. gingivalis. A chemotaxis assay was performed using a 48 well chemotaxis chamber. Chemotactic functional activity of THP-1 cells was increased 2-fold after 4 days of treatment with GM-CSF. Stimulation of THP-1 cells with LPS of F. nucleatum or P. gingivalis significantly reduced the chemotactic activity indicating the maturation towards a fixed macrophage. There were functional variations (chemotaxis and phagocytosis) in THP-1 cells in response to LPS of oral microorganisms following stimulation with GM-CSF.
已利用人THP-1单核细胞系培养系统来观察补充粒细胞巨噬细胞集落刺激因子（GM-CSF）与口腔微生物脂多糖（LPS）对刺激单核细胞/巨噬细胞功能活性的影响。通过酚水提取法制备并鉴定了口腔微生物具核梭杆菌和牙龈卟啉单胞菌的LPS。使用FITC标记的酿酒酵母颗粒进行吞噬试验。在10%-11%的静息THP-1细胞中观察到吞噬功能活性。用具核梭杆菌或牙龈卟啉单胞菌的LPS处理THP-1细胞可使THP-1细胞的吞噬活性提高2至3倍。GM-CSF单独使用或与具核梭杆菌或牙龈卟啉单胞菌的LPS一起补充时均显著增加吞噬作用。使用48孔趋化室进行趋化试验。用GM-CSF处理4天后，THP-1细胞的趋化功能活性提高了2倍。用具核梭杆菌或牙龈卟啉单胞菌的LPS刺激THP-1细胞显著降低趋化活性，表明其向固定巨噬细胞成熟。GM-CSF刺激后，THP-1细胞对口腔微生物LPS的反应存在功能差异（趋化和吞噬）。
