鲤鱼（鲤属）肝胰腺乳酸脱氢酶（EC 1.1.1.27）的纯化
Purification of carp (Cyprinus carpio) hepatopancreatic lactate dehydrogenase (EC 1.1.1.27).
作者信息
Kminková M, Kucera J
机构信息
Food Research Institute, Prague, Czech Republic.
出版信息
Prep Biochem Biotechnol. 1998 Nov;28(4):313-7. doi: 10.1080/10826069808010144.
PMID:9805350
Abstract
Lactate dehydrogenase (LDH, EC 1.1.1.27) was isolated in 86% purity from carp (Cyprinus carpio) hepatopancreas using agarose T-gel (mercaptoethanol bound to agarose via divinylsulphone) affinity chromatography medium and Iontosorb phenyl hydrophobic chromatography medium. Gel filtration chromatography of the LDH obtained shows molecular weight to be 26,900 Da.
摘要
利用琼脂糖T凝胶（巯基乙醇通过二乙烯砜与琼脂糖结合）亲和层析介质和Iontosorb苯基疏水层析介质，从鲤鱼（Cyprinus carpio）肝胰腺中分离出纯度为86%的乳酸脱氢酶（LDH，EC 1.1.1.27）。对所得乳酸脱氢酶进行凝胶过滤层析，结果显示其分子量为26,900道尔顿。
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