Harada N, Maeda M
Department of Biochemistry, Saitama University Urawa, Japan.
Biosci Biotechnol Biochem. 1998 Sep;62(9):1647-52. doi: 10.1271/bbb.62.1647.
Rhamnan sulfate was extracted with boiling water from the cell wall of Monostroma nitridum, and the resulting extract was purified by ion-exchange and size-exclusion chromatography. The polysaccharide, which is regarded as a homopolysaccharide, was 6-fold more antithrombin-active than the heparin standard. The antithrombin activity was decreased, by desulfation, although the non-active product still retained 8% of the sulfate ester. A comparative structural study of the intact rhamnan sulfate and rhamnan, its desulfated product, by periodate oxidation, Smith degradation and methylation analysis revealed the rhamnan sulfate to consist of alpha-1,3-linked L-rhamnose residues, some of which were substituted with sulfate groups mainly at position O-2. Minor amounts of internal 1,2-linked rhamnose and branched rhamnose linkages were also detected.
从绿紫菜细胞壁中用沸水提取硫酸鼠李聚糖，所得提取物通过离子交换和尺寸排阻色谱法进行纯化。该多糖被认为是一种同多糖，其抗凝血酶活性比肝素标准品高6倍。通过脱硫，抗凝血酶活性降低，尽管无活性产物仍保留8%的硫酸酯。通过高碘酸盐氧化、史密斯降解和甲基化分析对完整的硫酸鼠李聚糖及其脱硫产物鼠李聚糖进行的比较结构研究表明，硫酸鼠李聚糖由α-1,3-连接的L-鼠李糖残基组成，其中一些主要在O-2位被硫酸基团取代。还检测到少量内部1,2-连接的鼠李糖和分支鼠李糖键。
