Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
Bioresour Technol. 2012 Jun;114:414-8. doi: 10.1016/j.biortech.2012.03.025. Epub 2012 Mar 14.
A low molecular weight fraction, designated LMWP, was prepared by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum and purified by anion-exchange and gel-permeation chromatography. Chemical and spectroscopic analyses showed that LMWP was mainly composed of rhamnose, and its molecular weight was about 33.6 kDa. The backbone of LMWP consists of 1,3-linked α-L-rhamnose units with partially sulfate groups at the C-2 position. Approximately 25% of 1,3-linked α-L-rhamnose units is substituted at C-2 by sulfated or non-sulfated 1,3-linked α-L-rhamnose and 1,2-linked α-L-rhamnose units. LMWP effectively prolonged clotting time as evaluated by the activated partial thromboplastin time assay and was a potent thrombin inhibitor mediated by heparin cofactor II. The investigation demonstrated that LMWP is a novel sulfated polysaccharide with anticoagulant activity.
一种低分子量部分，命名为 LMWP，通过温和酸水解从 Monostroma latissimum 中的硫酸化鼠李聚糖制备，并通过阴离子交换和凝胶渗透色谱进行纯化。化学和光谱分析表明，LMWP 主要由鼠李糖组成，分子量约为 33.6 kDa。LMWP 的骨架由 1,3 连接的α-L-鼠李糖单元组成，部分硫酸基团位于 C-2 位置。大约 25%的 1,3 连接的α-L-鼠李糖单元在 C-2 处被硫酸化或非硫酸化的 1,3 连接的α-L-鼠李糖和 1,2 连接的α-L-鼠李糖单元取代。LMWP 通过活化部分凝血活酶时间测定有效延长凝血时间，是肝素辅因子 II 介导的有效凝血酶抑制剂。该研究表明，LMWP 是一种具有抗凝活性的新型硫酸化多糖。
