Nishida K, Ohta Y, Ishiguro I
Department of Biochemistry, School of Medicine, Fujita Health University, Toyoake, Aichi, 470-1192, Japan.
Pharmacol Res. 1998 Nov;38(5):393-400. doi: 10.1006/phrs.1998.0379.
A decrease in constitutive nitric oxide synthase (cNOS) activity occurred with decreases in hexosamine (an index of mucus synthesis) and adherent mucus (an index of mucus secretion) concentrations in the gastric mucosa of rats with water immersion restraint (WIR) stress. The decreases in gastric mucosal hexosamine and adherent mucus concentrations were enhanced with a further decrease in gastric mucosal cNOS activity by pretreatment with NG-monomethyl L-arginine, a non-selective NOS inhibitor, but were prevented with maintenance of the gastric mucosal cNOS activity by pretreatment with aminoguanidine, a selective inducible NO synthase inhibitor. In all WIR-stressed rats used in this study, gastric mucosal cNOS activity was well correlated with either gastric mucosal hexosamine or adherent mucus concentration (r=0.717 or 0.739, respectively). These results indicate that in the gastric mucosa of WIR-stressed rats, a decrease in cNOS activity is closely related to a decrease in mucus level due to impairment of its synthesis and secretion. (c) 1998 The Italian Pharmacological Society.
在水浸束缚（WIR）应激大鼠的胃黏膜中，组成型一氧化氮合酶（cNOS）活性降低，同时己糖胺（黏液合成指标）和黏附性黏液（黏液分泌指标）浓度也降低。用非选择性一氧化氮合酶抑制剂NG-单甲基-L-精氨酸预处理，可使胃黏膜cNOS活性进一步降低，进而增强胃黏膜己糖胺和黏附性黏液浓度的降低；而用选择性诱导型一氧化氮合酶抑制剂氨基胍预处理，维持胃黏膜cNOS活性，则可防止这种降低。在本研究中使用的所有WIR应激大鼠中，胃黏膜cNOS活性与胃黏膜己糖胺或黏附性黏液浓度均密切相关（r分别为0.717和0.739）。这些结果表明，在WIR应激大鼠的胃黏膜中，cNOS活性降低与黏液水平降低密切相关，这是由于其合成和分泌受损所致。（c）1998年意大利药理学会。
