Abd El-Rady Nessren M, Dahpy Marwa A, Ahmed Amel, Elgamal Dalia A, Hadiya Safy, Ahmed Mai A M, Sayed Zain El-Abdeen Ahmed, Abdeltawab Doaa, Abdelmohsen Ahmed Shawkat, Farrag Alshaimaa Abdelkhaliq Mohammad, Ashmawy Ahmed M, Khairallah Marwa K, Galal Heba M
Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Medical Physiology Department, Sphinx University, New Assiut, Egypt.
Front Physiol. 2021 Mar 23;12:649462. doi: 10.3389/fphys.2021.649462. eCollection 2021.
Few data are available about the role of herbal extract loaded nanoparticles as an alternative safe medicine for the management of a gastric ulcer.
This work is targeted at exploring the physiological effects of pomegranate loaded nanoparticles (PLN) against an indomethacin IND-induced gastric ulcer and comparing the results with traditional pomegranate peel extract (PPE).
Twenty-four rats were equally distributed into four groups: control, IND-treated, PLN-treated, and PPE-treated groups. Gross examination of gastric mucosa, and the calculation of ulcer and inhibition indices were done. Serum malondialdehyde (MDA), total antioxidant capacity (TAC), interleukin 2 (IL-2), IL-6, IL-10, gastric homogenate prostaglandin E (PGE), and nitric oxide (NO) were estimated. Mucosal endothelial nitric oxide synthase (eNOS mRNA) expression was identified by qPCR. Histological and immuno-histochemical staining of Tumor necrosis factor-α (TNF-α) and eNOS of stomach mucosa were performed.
In comparison with the control group, IND-treated rats showed visible multiple ulcers with ulcer index, serum MDA, IL-2 and IL-6 were elevated while IL-10, PGE, NO, and eNOS mRNA expression were significantly reduced. Damaged surface epithelium with disrupted glandular architecture and heavy leucocyte infiltration of lamina propria was noticed. Immunohistochemical staining of stomach mucosa revealed marked increased TNF-α and reduced eNOS. Oral administration of PLN and PPE succeeded in improving the gross mucosal picture, and all biochemical, histological, and immunohistochemical alterations.
Both PLN and PPE potently alleviated IND-induced gastric ulceration increasing TAC, PGE, NO, eNOS mRNA, and protein expression. However, the healing effect of PLN was obviously greater than PPE-treated rats.
关于负载草药提取物的纳米颗粒作为治疗胃溃疡的替代安全药物的作用，可用数据较少。
本研究旨在探讨负载石榴籽的纳米颗粒（PLN）对吲哚美辛（IND）诱导的胃溃疡的生理作用，并将结果与传统石榴皮提取物（PPE）进行比较。
将24只大鼠平均分为四组：对照组、IND治疗组、PLN治疗组和PPE治疗组。对胃黏膜进行大体检查，并计算溃疡指数和抑制指数。测定血清丙二醛（MDA）、总抗氧化能力（TAC）、白细胞介素2（IL-2）、IL-6、IL-10、胃匀浆前列腺素E（PGE）和一氧化氮（NO）。通过qPCR鉴定黏膜内皮一氧化氮合酶（eNOS mRNA）表达。对胃黏膜肿瘤坏死因子-α（TNF-α）和eNOS进行组织学和免疫组织化学染色。
与对照组相比，IND治疗的大鼠出现明显的多处溃疡，溃疡指数、血清MDA、IL-2和IL-6升高，而IL-10、PGE、NO和eNOS mRNA表达显著降低。观察到表面上皮受损，腺结构破坏，固有层有大量白细胞浸润。胃黏膜免疫组织化学染色显示TNF-α明显增加，eNOS减少。口服PLN和PPE成功改善了大体黏膜情况，以及所有生化、组织学和免疫组织化学改变。
PLN和PPE均能有效减轻IND诱导的胃溃疡，增加TAC、PGE、NO、eNOS mRNA和蛋白表达。然而，PLN的愈合效果明显优于PPE治疗的大鼠。
