Zhang J S, Nelson M, Wang L, Liu W, Qian C P, Shridhar V, Urrutia R, Smith D I
Department of Laboratory Medicine and Pathology, Mayo Foundation, Rochester, Minnesota, 55905, USA.
Genomics. 1998 Nov 15;54(1):149-54. doi: 10.1006/geno.1998.5458.
Sodium butyrate (NaB) was shown to induce growth inhibition and apoptosis in a human pancreatic cancer cell line (AsPC-1). To identify the genes that are differentially regulated by NaB, we generated subtracted EST libraries highly enriched for up- or down-regulated transcripts using the suppression subtraction hybridization technique. One of the ESTs identified in the down-regulated library showed significant homology to human alpha-catenin. A cDNA of 2.45 kb that codes for a putative protein of 734 aa was cloned. The cloned cDNA was used as a template in an in vitro transcription-translation reaction. This produced a protein product of about 82 kDa in size. The gene designated as CTNNAL1 (catenin (cadherin-associated protein), alpha-like 1) was found to be ubiquitously expressed in many tissues including pancreas, heart, and skeletal muscle. A human BAC clone containing the gene was isolated and used as a probe for fluorescence in situ hybridization (FISH). Both radiation hybrid and FISH analysis mapped the gene to chromosome band 9q31.2, a region where frequent abnormalities have been observed in bladder carcinoma, esophageal cancers, and several other tumors.
丁酸钠（NaB）已被证明可诱导人胰腺癌细胞系（AsPC-1）生长抑制和凋亡。为了鉴定受NaB差异调节的基因，我们使用抑制性消减杂交技术构建了高度富集上调或下调转录本的消减EST文库。在下调文库中鉴定出的一个EST与人类α-连环蛋白具有显著同源性。克隆了一个2.45 kb的cDNA，其编码一个734个氨基酸的假定蛋白。克隆的cDNA用作体外转录-翻译反应的模板。这产生了一个大小约为82 kDa的蛋白质产物。发现命名为CTNNAL1（连环蛋白（钙黏着蛋白相关蛋白），α样1）的基因在包括胰腺、心脏和骨骼肌在内的许多组织中普遍表达。分离出一个包含该基因的人BAC克隆，并用作荧光原位杂交（FISH）的探针。辐射杂种和FISH分析均将该基因定位于9q31.2染色体带，在膀胱癌、食管癌和其他几种肿瘤中经常观察到该区域存在异常。
