Gontijo N F, Almeida-Silva S, Costa F F, Mares-Guia M L, Williams P, Melo M N
Departamento de Parasitologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais-UFMG, Caixa Postal 486, Minas Gerais, Belo Horizonte, CEP 31 270-901, Brazil.
Exp Parasitol. 1998 Nov;90(3):212-9. doi: 10.1006/expr.1998.4336.
Screening for digestive glycosidases in different parts of the gut and associated organs of Lutzomyia longipalpis is reported. Searches for the enzymes were made in blood-fed and non-blood-fed females and the enzymes were characterized as soluble or membrane-bound molecules. A total of four different activities were detected, corresponding to the following specificities: an alpha-glucosidase, an N-acetyl-beta-d-glucosaminidase, an N-acetyl-beta-d-galactosaminidase, and an alpha-l-fucosidase. Their possible role and importance for Leishmania development are discussed and the alpha-glucosidase enzyme was partially characterized. The pH inside the gut of non-blood-fed phlebotomines was measured with pH indicator dyes. The pH ranges obtained for crop, midgut, and hindgut were, respectively, higher than pH 6, pH 6, and lower than pH 6. A hypothesis concerning these data and Leishmania development is proposed.
本文报道了对长须罗蛉肠道及相关器官不同部位消化糖苷酶的筛选。在吸食血液和未吸食血液的雌性罗蛉中寻找这些酶，并将其鉴定为可溶性或膜结合分子。总共检测到四种不同的活性，分别对应以下特异性：一种α-葡萄糖苷酶、一种N-乙酰-β-D-葡萄糖胺酶、一种N-乙酰-β-D-半乳糖胺酶和一种α-L-岩藻糖苷酶。讨论了它们对利什曼原虫发育的可能作用和重要性，并对α-葡萄糖苷酶进行了部分特性鉴定。使用pH指示剂染料测量了未吸食血液的白蛉肠道内的pH值。嗉囊、中肠和后肠的pH范围分别高于pH 6、pH 6和低于pH 6。提出了一个关于这些数据和利什曼原虫发育的假说。
