一种与软骨细胞膜相关的聚集蛋白聚糖降解活性。
An aggrecan-degrading activity associated with chondrocyte membranes.
作者信息
Billington C J, Clark I M, Cawston T E
机构信息
Department of Rheumatology, School of Clinical and Medical Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, UK.
出版信息
Biochem J. 1998 Nov 15;336 ( Pt 1)(Pt 1):207-12. doi: 10.1042/bj3360207.
Abstract
The breakdown of aggrecan in cartilage is, in part, mediated by an enzyme named aggrecanase that cleaves within the interglobular domain of the molecule between a glutamic residue and an alanine residue. Although the enzyme cleavage site has been identified, the identity, characteristics and localization of this enzyme remain unclear. We have demonstrated that membranes isolated from stimulated chondrocytes are able to generate aggrecan fragments that are labelled by an antibody that recognizes the new N-terminus formed by aggrecanase activity. It was further shown that the membrane activity was a metalloproteinase but was not inhibited by the naturally occurring matrix metalloproteinase (MMP) inhibitors, TIMPs 1 and 2. These results show that an aggrecanase activity is associated with the membranes of the chondrocytes and is a metalloproteinase, but might not be a member of the MMP family.
摘要
软骨中聚集蛋白聚糖的降解部分是由一种名为聚集蛋白聚糖酶的酶介导的，该酶在分子的球间结构域内谷氨酸残基和丙氨酸残基之间进行切割。尽管已经确定了该酶的切割位点，但这种酶的身份、特性和定位仍不清楚。我们已经证明，从受刺激的软骨细胞中分离出的膜能够产生聚集蛋白聚糖片段，这些片段可被识别由聚集蛋白聚糖酶活性形成的新N端的抗体标记。进一步表明，膜活性是一种金属蛋白酶，但不受天然存在的基质金属蛋白酶（MMP）抑制剂TIMP 1和TIMP 2的抑制。这些结果表明，聚集蛋白聚糖酶活性与软骨细胞膜相关，是一种金属蛋白酶，但可能不是MMP家族的成员。
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本文引用的文献
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The interglobular domain of cartilage aggrecan is cleaved by hemorrhagic metalloproteinase HT-d (atrolysin C) at the matrix metalloproteinase and aggrecanase sites.软骨聚集蛋白聚糖的球间结构域在基质金属蛋白酶和聚集蛋白聚糖酶作用位点被出血性金属蛋白酶HT-d（抗胰蛋白酶C）切割。
J Biol Chem. 1998 Mar 6;273(10):5846-50. doi: 10.1074/jbc.273.10.5846.
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Utilization of a recombinant substrate rAgg1 to study the biochemical properties of aggrecanase in cell culture systems.
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Membrane protein secretases.膜蛋白分泌酶
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Biochem Biophys Res Commun. 1997 Jan 13;230(2):335-9. doi: 10.1006/bbrc.1996.5957.
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Degradation of cartilage aggrecan by collagenase-3 (MMP-13).胶原蛋白酶-3（基质金属蛋白酶-13）对软骨聚集蛋白聚糖的降解作用。
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