Zeller W, Weber H, Panoussis B, Bürge T, Bergmann R
Novartis Pharma Ltd, Basel, Switzerland.
Lab Anim. 1998 Oct;32(4):369-76. doi: 10.1258/002367798780599910.
A refined method of repeated blood sampling is described: the tongue of the anaesthetized rat is pulled forward with the fingers and the sublingual vein is punctured with a 23 gauge hypodermic needle. Based on the requirement of a pharmacokinetic study, 0.5 or 1 ml of blood was collected 7 times at 0, 0.5, 1, 2, 4, 8 and 24 h. The degree of suffering was judged by determining the body weight and food and water consumption. All animals showed an increase in body weight already after 24 h and, therefore, the method of collecting blood from the sublingual vein can be recommended for repeated blood sampling. The haematological evaluation of groups of animals with differing body weight showed that sample volumes of up to 15% of the total blood volume lead to haematocrit values of approximately 40%. A remarkable initial drop in white blood cell counts followed by a marked rise 2 h after first sampling to values partly above the pre-test could not be directly related to the extracted blood volume.
用手指将麻醉大鼠的舌头向前拉，然后用23号皮下注射针穿刺舌下静脉。根据药代动力学研究的要求，在0、0.5、1、2、4、8和24小时分别采集7次血样，每次采集0.5或1毫升。通过测定体重以及食物和水的消耗量来判断痛苦程度。所有动物在24小时后体重均有所增加，因此，舌下静脉采血法可推荐用于重复采血。对不同体重动物组的血液学评估表明，采血量高达总血量的15%时，血细胞比容值约为40%。首次采血后白细胞计数显著下降，随后在2小时时显著上升，部分值高于测试前，这与采血量无直接关系。
