Mahl A, Heining P, Ulrich P, Jakubowski J, Bobadilla M, Zeller W, Bergmann R, Singer T, Meister L
Novartis Pharma AG, Preclinical Safety: Toxicology/Pathology, Basel, Switzerland.
Lab Anim. 2000 Oct;34(4):351-61. doi: 10.1258/002367700780387787.
Blood samples were taken from the retrobulbar venous plexus or the sublingual vein of male HamIbm:Wist rats to compare clinical pathology parameters between the two sampling techniques. By analogy with a pharmacokinetic study, blood was sampled six times during one day from unfasted animals. After 3 weeks of recovery, blood was taken from fasted animals on a single occasion. In addition, prolactin and corticosterone levels were determined to compare stress-related effects between the two sampling methods. Body weight development and food consumption were similar after single as well as after repeated blood sampling for the two blood sampling techniques. Haemotological evaluation showed a gradual decrease in erythrocyte count, haemoglobin concentration and haematocrit after repeated blood sampling. Repeated withdrawal of blood samples over 24 h corresponding to approximately 22% of the total blood volume resulted in a decrease in red blood cell parameters by up to 30%. The withdrawal of approximately 10% of the total blood volume was associated with a decrease in these parameters by up to 10% and should not be exceeded for animal welfare reasons and to allow a reliable evaluation of data in a study. Repeated blood sampling was associated with an initial decrease in the number of white blood cells, mainly due to a reduction in lymphocytes; white blood cell counts were slightly increased one day after. The decrease in lymphocytes and the increase in neutrophils after repeated sampling were generally slightly more pronounced in the blood from the retrobulbar plexus than from the sublingual vein. Comparison of serum clinical chemistry data showed significantly higher activities of creatine kinase and aspartate aminotransferase in samples from the retrobulbar plexus. These findings suggest a higher degree of tissue damage with blood sampling from the retrobulbar plexus than from the sublingual vein. Despite a large inter-individual variability, higher mean values of prolactin on each occasion and corticosterone after a single sample in fasted animals indicate a higher stress associated with blood sampling from the retrobulbar plexus.
从雄性HamIbm:Wist大鼠的球后静脉丛或舌下静脉采集血样，以比较两种采血技术之间的临床病理参数。类似于药代动力学研究，在一天内对未禁食的动物进行六次采血。恢复3周后，对禁食动物进行一次采血。此外，测定催乳素和皮质酮水平，以比较两种采血方法之间与应激相关的影响。对于两种采血技术，单次采血和重复采血后，体重增长和食物消耗情况相似。血液学评估显示，重复采血后红细胞计数、血红蛋白浓度和血细胞比容逐渐下降。在24小时内重复采血相当于总血容量的约22%，导致红细胞参数下降高达30%。抽取约10%的总血容量会使这些参数下降高达10%，出于动物福利原因并为了在研究中能够可靠地评估数据，不应超过此量。重复采血最初会导致白细胞数量减少，主要是由于淋巴细胞减少；一天后白细胞计数略有增加。重复采血后淋巴细胞减少和中性粒细胞增加的情况，在球后丛血液中通常比舌下静脉血液中更为明显。血清临床化学数据比较显示，球后丛血样中肌酸激酶和天冬氨酸转氨酶的活性显著更高。这些发现表明，从球后丛采血比从舌下静脉采血对组织的损伤程度更高。尽管个体间存在较大差异，但禁食动物单次采血后每次催乳素和皮质酮的平均数值较高，表明从球后丛采血与更高的应激相关。
