Bramlage B, Luzi E, Eckstein F
Max-Planck-Institut für Experimentelle Medizin, Göttingen, Germany.
Trends Biotechnol. 1998 Oct;16(10):434-8. doi: 10.1016/s0167-7799(98)01236-0.
Ribozymes are being increasingly used for the sequence-specific inhibition of gene expression by the cleavage of mRNAs encoding proteins of interest. However, particular attention must be paid to the following points: the identification of regions on the mRNA accessible to the ribozyme; the delivery of ribozymes to cells by either exogenous or endogenous delivery; colocalization of the ribozyme with the target RNA in the cell; and differentiation between closely related sequences. This field is advancing rapidly, and results obtained with transgenic animals demonstrate the power of this strategy for the inhibition of gene expression.
核酶正越来越多地用于通过切割编码目标蛋白质的mRNA来对基因表达进行序列特异性抑制。然而，必须特别注意以下几点：确定mRNA上可被核酶作用的区域；通过外源性或内源性递送将核酶递送至细胞；使核酶与细胞内的靶RNA共定位；以及区分密切相关的序列。该领域发展迅速，转基因动物实验所获得的结果证明了这种抑制基因表达策略的强大作用。
