用于补体结合试验的口蹄疫病毒在牛白细胞培养物中的培养
Cultivation of foot and mouth disease virus in bovine leukocyte cultures for use in the complement fixation test.
作者信息
Rai A
出版信息
Acta Virol. 1976 Jun;20(3):257-9.
PMID:9808
Abstract
Bovine leukocyte cultures achieved good growth after 72 hours of cultivation. Foot-and mouth disease (FMD) virus type 0 multiplied in such cultures to a titre of 10(6) TCID50/0.1 ml. The cell culture-grown virus was found to be suitable for the complement fixation test (CFT) after purification and concentration with calcium phosphate.
摘要
牛白细胞培养物在培养72小时后生长良好。0型口蹄疫病毒在此类培养物中增殖，效价达到10(6) TCID50/0.1毫升。经磷酸钙纯化和浓缩后，发现细胞培养生长的病毒适用于补体结合试验（CFT）。
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