Maestroni G J, Cosentino M, Marino F, Togni M, Conti A, Lecchini S, Frigo G
Center for Experimental Pathology, Cantonal Institute of Pathology, Locarno, Switzerland.
Exp Hematol. 1998 Nov;26(12):1172-7.
Members of our research team have recently reported that adrenergic agents may affect hematopoiesis via alpha1-adrenoceptors present on bone marrow B cell precursors. In this paper we demonstrate that murine bone marrow contains a substantial amount of catecholamines. Norepinephrine (NE) and dopamine (DA) exhibited a daily rhythmicity, with peak values observed during the night. The rhythm was disrupted by chemical sympathectomy, whereas epinephrine (E) showed no rhythmicity or sensitivity to 6-hydroxydopamine. High and low values of NE and DA were associated with high and low values of their metabolites, which indicated a rhythmic catecholamine release. NE, but not DA or E, was positively associated with the proportion of cells in the G2/M and S phases of the cell cycle. Moreover, NE and DA were found in both short-term and long-term bone marrow cultures as well as in human or murine B lymphoid cell lines. These findings indicate that endogenous catecholamines in the bone marrow have both neural and cellular origins. The neural input shows a daily rhythm and may be implicated in the regulation of hematopoiesis.
我们研究团队的成员最近报告称，肾上腺素能药物可能通过存在于骨髓B细胞前体上的α1-肾上腺素受体影响造血作用。在本文中，我们证明小鼠骨髓中含有大量儿茶酚胺。去甲肾上腺素（NE）和多巴胺（DA）呈现出每日节律性，在夜间观察到峰值。这种节律被化学交感神经切除术破坏，而肾上腺素（E）未表现出节律性或对6-羟基多巴胺的敏感性。NE和DA的高值和低值与其代谢物的高值和低值相关，这表明儿茶酚胺释放具有节律性。NE与细胞周期G2/M期和S期的细胞比例呈正相关，而DA或E则不然。此外，在短期和长期骨髓培养物以及人或小鼠B淋巴细胞系中均发现了NE和DA。这些发现表明，骨髓中的内源性儿茶酚胺具有神经和细胞来源。神经输入呈现每日节律，可能参与造血作用的调节。
