Sakurai M, Fukuyama N, Takizawa S, Abe K, Hayashi T, Shinohara Y, Nakazawa H, Tabayashi K
Department of Thoracic and Cardiovascular Surgery, Tohoku University School of Medicine, Sendai, Japan.
J Cereb Blood Flow Metab. 1998 Nov;18(11):1233-8. doi: 10.1097/00004647-199811000-00009.
The induction and distribution of 3-L-nitrotyrosine (NO2-Tyr) were examined with HPLC and immunohistochemistry in rabbit spinal cords after 15 minutes of transient ischemia until 7 days of the reperfusion. After the 15-minute ischemia, there was a significant decrease of neurologic scores in the ischemic group compared with the sham-operated control group at 7 days of reperfusion (P = 0.0017), and the majority of motor neurons was selectively lost at 7 days of reperfusion (P = 0.0039). NO2-Tyr was transiently induced at 8 hours of reperfusion in the ventral part of the spinal cord (0.47%+/-0.86%, NO2-Tyr/total tyrosine; P = 0.0021), but was not induced at any time point of reperfusion in the dorsal part of the spinal cord. Strong immunoreactivity for NO2-Tyr was selectively induced in large pyramidal motor neurons at 8 hours of reperfusion and was still weakly present until 7 days of reperfusion. (There may be a difference in sensitivity between the two techniques.) These results suggested that protein tyrosine nitration by nitric oxide plays a role in the selective motor neuron cell damage after transient spinal cord ischemia.
在兔脊髓短暂缺血15分钟直至再灌注7天的过程中，采用高效液相色谱法（HPLC）和免疫组织化学方法检测3-L-硝基酪氨酸（NO2-Tyr）的诱导及分布情况。缺血15分钟后，再灌注7天时，缺血组神经功能评分与假手术对照组相比显著降低（P = 0.0017），且再灌注7天时大多数运动神经元选择性丢失（P = 0.0039）。再灌注8小时时，脊髓腹侧NO2-Tyr短暂诱导（0.47%±0.86%，NO2-Tyr/总酪氨酸；P = 0.0021），但脊髓背侧再灌注的任何时间点均未诱导。再灌注8小时时，大的锥体运动神经元中选择性诱导出强烈的NO2-Tyr免疫反应性，直至再灌注7天时仍微弱存在。（两种技术之间可能存在敏感性差异。）这些结果表明，一氧化氮介导的蛋白质酪氨酸硝化在短暂脊髓缺血后选择性运动神经元细胞损伤中起作用。
