Yokoyama Y, Takahashi Y, Shinohara A, Lian Z, Xiaoyun W, Niwa K, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu, Japan.
Mol Hum Reprod. 1998 Oct;4(10):985-9. doi: 10.1093/molehr/4.10.985.
Telomerase activity is associated with the proliferative activity of cells. In the endometrium, telomerase activity is higher in the proliferative phase than in the secretory phase of the menstrual cycle, suggesting that telomerase activity may occur primarily in the glandular epithelial cells. To test this, a dissociated cell culture of the endometrium was performed, and the telomerase activity in each cell fraction was analysed. Telomerase activity was found in all 10 endometrial tissues of the proliferative phase of the menstrual cycle. Both the fragments of epithelial glands and single cells, which were prepared by enzymatic dissociation, showed telomerase activity. In the 7 day cell culture, it was found in nine out of 10 epithelial cell enriched fractions, but in none of the stromal cell enriched fractions. Flow cytometric analysis showed that the epithelial enriched fraction was contaminated with a predominant number of stromal cells, while the stromal cell enriched fraction was comprised mostly of stromal cells with apparent proliferative activity. Our results suggest that telomerase activity of the endometrium occurs primarily in the epithelial cells in the endometrium and that the stromal cells do not express telomerase activity regardless of their potent proliferative activity.
端粒酶活性与细胞的增殖活性相关。在子宫内膜中，月经周期增殖期的端粒酶活性高于分泌期，这表明端粒酶活性可能主要发生在腺上皮细胞中。为了验证这一点，进行了子宫内膜的解离细胞培养，并分析了每个细胞组分中的端粒酶活性。在月经周期增殖期的所有10个子宫内膜组织中均发现了端粒酶活性。通过酶解制备的上皮腺体片段和单个细胞均显示出端粒酶活性。在7天的细胞培养中，在10个富含上皮细胞的组分中有9个发现了端粒酶活性，但在富含基质细胞的组分中均未发现。流式细胞术分析表明，富含上皮细胞的组分被大量基质细胞污染，而富含基质细胞的组分主要由具有明显增殖活性的基质细胞组成。我们的结果表明，子宫内膜的端粒酶活性主要发生在内膜的上皮细胞中，并且基质细胞无论其增殖活性如何都不表达端粒酶活性。
