Corwin R L, Rice H B
Nutrition Department, College of Health and Human Development, The Pennsylvania State University, University Park 16802, USA.
Physiol Behav. 1998 Aug;65(1):1-10. doi: 10.1016/s0031-9384(98)00078-x.
The peptide enterostatin has been proposed to function as a selective signal for fat-induced satiety. In the majority of enterostatin studies, however, rats were food-deprived, and the test food was also the maintenance diet. The present study sought to determine if enterostatin would selectively reduce consumption of oil that was provided in addition to a standard diet in non-food-deprived rats. Rats had either continuous (24-h/day) or limited access (120-min/day) to either a 32% sucrose solution or 100% corn oil. In addition to the sucrose and the oil, rats also had 22-h access to a standard pelleted rodent diet. Control rats had unlimited access to the standard diet but no access to oil or sucrose. Rats were maintained on their respective diets for 3 weeks before enterostatin testing. Food intake and body weight were monitored. Rats with continuous access to oil or sucrose consumed more calories and gained more weight than control rats. Caloric intake and body weight of the rats with limited access to oil or sucrose did not differ significantly from controls. Enterostatin, administered intraperitoneally (i.p.) at doses of 0 (vehicle), 89, 178, and 356 microg/kg, had no effect on consumption of oil, sucrose, or standard diet in these non-food-deprivation paradigms; however, 356 microg/kg reduced standard-diet intake when rats were overnight food-deprived, thus verifying peptide activity. These results do not support a role for enterostatin in the regulation of fat intake when optional high-fat foods are provided in addition to a readily available standard diet.
肽类肠抑素被认为是脂肪诱导饱腹感的一种选择性信号。然而，在大多数肠抑素研究中，大鼠处于食物剥夺状态，且测试食物也是维持饮食。本研究旨在确定肠抑素是否会选择性降低非食物剥夺状态下大鼠在标准饮食之外额外提供的油的摄入量。大鼠可以持续（每天24小时）或有限制地（每天120分钟）获取32%的蔗糖溶液或100%的玉米油。除了蔗糖和油之外，大鼠还可以在22小时内获取标准颗粒状啮齿动物饮食。对照大鼠可以无限制地获取标准饮食，但无法获取油或蔗糖。在进行肠抑素测试前，大鼠按各自的饮食喂养3周。监测食物摄入量和体重。持续获取油或蔗糖的大鼠比对照大鼠消耗更多热量且体重增加更多。有限制地获取油或蔗糖的大鼠的热量摄入量和体重与对照大鼠相比无显著差异。以0（赋形剂）、89、178和356微克/千克的剂量腹腔注射肠抑素，在这些非食物剥夺范式中，对油、蔗糖或标准饮食的摄入量没有影响；然而，当大鼠隔夜食物剥夺时，356微克/千克的剂量会降低标准饮食摄入量，从而验证了该肽的活性。当除了随时可得的标准饮食外还提供可选的高脂肪食物时，这些结果不支持肠抑素在调节脂肪摄入方面的作用。
