Illarionova R P, Bondarenko B N, Parkhomenko L V, Bernasovskaia E P
Prikl Biokhim Mikrobiol. 1976 Sep-Oct;12(5):784-7.
The paper presents a method for quantitative assay of lecithinases and lipases in microorganisms. The method is based on the assessment of changes in the composition of lipid components of yolk media under the influence of corresponding enzymic systems of bacteria. The technique involved lipid extraction from yolk media after cultivation of the microorganisms tested, chromatographic separation by thin-layer chromatography on silicagel into individual fractions, development in iodine vapours and quantitative measurement of lipid components.
本文介绍了一种定量测定微生物中卵磷脂酶和脂肪酶的方法。该方法基于评估蛋黄培养基脂质成分在细菌相应酶系统作用下的变化。该技术包括在测试微生物培养后从蛋黄培养基中提取脂质，通过硅胶薄层层析进行色谱分离成单个组分，在碘蒸气中显色以及对脂质成分进行定量测量。
