Nakai K, Fusazaki T, Zhang T, Shiroto T, Osawa M, Kamata J, Itoh M, Nakai K, Habano W, Kiuchi T, Yamamori S, Hiramori K
Second Department of Internal Medicine, Iwate Medical University, Morioka, Japan.
Coron Artery Dis. 1998;9(6):329-34. doi: 10.1097/00019501-199809060-00002.
To investigate the genetic contribution for myocardial infarction.
We investigated common polymorphisms of apolipoprotein E gene and angiotensin converting enzyme (ACE) gene in Japanese population. Subjects were 422 healthy people and 254 patients with myocardial infarction. We evaluated the 287 base pair (bp) insertion (I)/deletion (D) polymorphism in intron 16 of the ACE gene and a polymorphism in the apolipoprotein E gene by using the polymerase chain reaction.
The ACE genotype prevalences for II, ID, and DD were 36.2, 46.1, and 17.7%, respectively, among the myocardial infarction patients. The prevalence of the D allele of the ACE gene among the myocardial infarction patients (0.593) exceeded that among the healthy controls (0.407). The prevalences of the epsilon 2, epsilon 3, and epsilon 4 alleles of the apolipoprotein E genotype among healthy controls were 0.024, 0.882, and 0.094, and those among survivors of myocardial infarction were 0.024, 0.834, and 0.142, respectively. Myocardial infarction patients had an excessive prevalence of the apolipoprotein E epsilon 4 allele (P < 0.05). Multiple regression analysis demonstrated that the independent risk factors for developing myocardial infarction were age, DD genotype of ACE gene, and apolipoprotein E epsilon 4 allele. Stenotic coronary vessels in myocardial infarction patients did not differ significantly among the patients with various ACE and apolipoprotein E genotypes in the present study.
Among the Japanese, apolipoprotein E epsilon 4 carriers and subjects with ACE DD genotype are at an increased risk of myocardial infarction.
研究心肌梗死的遗传因素。
我们在日本人群中调查了载脂蛋白E基因和血管紧张素转换酶（ACE）基因的常见多态性。研究对象为422名健康人和254名心肌梗死患者。我们采用聚合酶链反应评估了ACE基因第16内含子中的287碱基对（bp）插入（I）/缺失（D）多态性以及载脂蛋白E基因的多态性。
心肌梗死患者中ACE基因型II、ID和DD的患病率分别为36.2%、46.1%和17.7%。心肌梗死患者中ACE基因D等位基因的患病率（0.593）超过了健康对照组（0.407）。健康对照组中载脂蛋白E基因型的ε2、ε3和ε4等位基因的患病率分别为0.024、0.882和0.094，心肌梗死幸存者中的患病率分别为0.024、0.834和0.142。心肌梗死患者中载脂蛋白Eε4等位基因的患病率过高（P<0.05）。多元回归分析表明，发生心肌梗死的独立危险因素为年龄、ACE基因的DD基因型和载脂蛋白Eε4等位基因。在本研究中，不同ACE和载脂蛋白E基因型的心肌梗死患者的狭窄冠状动脉血管差异无统计学意义。
在日本人中，载脂蛋白Eε4携带者和ACE DD基因型受试者发生心肌梗死的风险增加。
