Matheson Z, Hargy T M, McCuin R M, Clancy J L, Fricker C R
Thames Water Utilities Ltd, Spencer House Laboratories, Reading, UK.
J Appl Microbiol. 1998 Oct;85(4):755-61. doi: 10.1111/j.1365-2672.1998.00588.x.
The Gelman Envirochek capsule is a membrane device for the simultaneous concentration of Cryptosporidium oocysts and Giardia cysts from water. Samples are filtered through a Supor polyethersulphone membrane with a 1 micron absolute pore size. (Oo)cysts are mechanically eluted from the membrane fibre using a wrist action shaker and a nonionic detergent and concentrated by centrifugation. The concentrate can be further processed using any separation technique to separate the target organisms from other debris. This method enables multiple samples to be processed within 1 h. Recoveries from seeded tap and source water samples were in excess of 70% for Cryptosporidium and 80% for Giardia.
盖尔曼环境检测胶囊是一种用于同时从水中浓缩隐孢子虫卵囊和贾第虫包囊的膜装置。样品通过孔径绝对为1微米的苏泊尔聚醚砜膜进行过滤。(卵)囊使用手腕式振荡器和非离子洗涤剂从膜纤维上机械洗脱,并通过离心进行浓缩。浓缩物可使用任何分离技术进一步处理,以将目标生物体与其他杂质分离。该方法能够在1小时内处理多个样品。对于接种的自来水和水源水样品,隐孢子虫的回收率超过70%,贾第虫的回收率超过80%。