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对Gelman Envirochek胶囊用于从水中同时浓缩隐孢子虫和贾第鞭毛虫的评估。

An evaluation of the Gelman Envirochek capsule for the simultaneous concentration of Cryptosporidium and Giardia from water.

作者信息

Matheson Z, Hargy T M, McCuin R M, Clancy J L, Fricker C R

机构信息

Thames Water Utilities Ltd, Spencer House Laboratories, Reading, UK.

出版信息

J Appl Microbiol. 1998 Oct;85(4):755-61. doi: 10.1111/j.1365-2672.1998.00588.x.

DOI:10.1111/j.1365-2672.1998.00588.x
PMID:9812387
Abstract

The Gelman Envirochek capsule is a membrane device for the simultaneous concentration of Cryptosporidium oocysts and Giardia cysts from water. Samples are filtered through a Supor polyethersulphone membrane with a 1 micron absolute pore size. (Oo)cysts are mechanically eluted from the membrane fibre using a wrist action shaker and a nonionic detergent and concentrated by centrifugation. The concentrate can be further processed using any separation technique to separate the target organisms from other debris. This method enables multiple samples to be processed within 1 h. Recoveries from seeded tap and source water samples were in excess of 70% for Cryptosporidium and 80% for Giardia.

摘要

盖尔曼环境检测胶囊是一种用于同时从水中浓缩隐孢子虫卵囊和贾第虫包囊的膜装置。样品通过孔径绝对为1微米的苏泊尔聚醚砜膜进行过滤。(卵)囊使用手腕式振荡器和非离子洗涤剂从膜纤维上机械洗脱,并通过离心进行浓缩。浓缩物可使用任何分离技术进一步处理,以将目标生物体与其他杂质分离。该方法能够在1小时内处理多个样品。对于接种的自来水和水源水样品,隐孢子虫的回收率超过70%,贾第虫的回收率超过80%。

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引用本文的文献

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Molecular characterization of Cryptosporidium isolates obtained from humans in France.法国人类隐孢子虫分离株的分子特征分析。
J Clin Microbiol. 2001 Oct;39(10):3472-80. doi: 10.1128/JCM.39.10.3472-3480.2001.
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Contamination of river water by Cryptosporidium parvum oocysts in western Japan.
日本西部河水受微小隐孢子虫卵囊污染情况。
Appl Environ Microbiol. 2001 Sep;67(9):3832-6. doi: 10.1128/AEM.67.9.3832-3836.2001.
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Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for recovering oocysts from water samples.采用膜滤溶解法从水样中回收微小隐孢子虫卵囊,体外降低微小隐孢子虫卵囊的感染性。
Appl Environ Microbiol. 2001 Jul;67(7):3309-13. doi: 10.1128/AEM.67.7.3309-3313.2001.