Huang W, Jiang H, Qiu J
Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 1997 Jun;11(6):249-51.
This experiment aimed to explore the effects of substance P (SP) on secretory otitis media (SOM). Both immunohistochemistry ABC-GDN and image pattern analysis technique were adopted to investigate the relation between SP content of SOM middle ear mucosa and middle ear effusion, and observe the effect of SP receptor antagonist spantide and histamine H2 receptor blocker cimetidin on middle ear effusion. The findings showed: middle ear mucosa SP content tended to increase, and had positive correlation with middle ear effusion, intra-abdominally injecting 1 mg/ml Spantide and 1 mg/ml Cinetidin per day could have middle ear effusion decreased obviously, but the quantities of reduction were more significant. The results suggest that SP plays a role in SOM, might accelerate vasodilation and increase the permeability of cupillary in middle ear mucosa mediated by histamine.
本实验旨在探讨P物质（SP）对分泌性中耳炎（SOM）的影响。采用免疫组织化学ABC - GDN法及图像模式分析技术，研究SOM中耳黏膜及中耳积液中SP含量的关系，并观察SP受体拮抗剂spantide和组胺H2受体阻滞剂西咪替丁对中耳积液的影响。结果显示：中耳黏膜SP含量有升高趋势，且与中耳积液呈正相关，每天腹腔注射1mg/ml spantide和1mg/ml西咪替丁可使中耳积液明显减少，但减少量更显著。结果提示，SP在SOM中起作用，可能通过组胺介导加速中耳黏膜血管舒张并增加毛细血管通透性。
