He S F, Xie Z
Shanghai Brain Research Institute, Chinese Academy of Sciences.
Sheng Li Xue Bao. 1997 Feb;49(1):31-8.
In the present study, the properties of voltage-dependent K- currents in cultured rat C6 glioma cells were investigated with the whole cell patch-clamp recording technique. A total of 97 cells were recorded. The mean value of the membrane potential was -47.5 +/- 9.4 mV. Seven percent of the recorded C6 glioma cells did not show any outward potassium currents. The rest had outward K+ currents which could be activated at -36.5 +/- 5.9 mV from a holding potential of -70 mV. Ananalysis of tail currents, showed the mean value of the reversal potential was -84.6 +/- 4.1 mV, close to the K+ equilibrium potential. The K+ currents of 60% of the recorded C6 cells consisted of both a fast and a slow component, while those of the 33% only with a slow component.
在本研究中，采用全细胞膜片钳记录技术研究了培养的大鼠C6胶质瘤细胞中电压依赖性钾电流的特性。共记录了97个细胞。膜电位的平均值为-47.5±9.4 mV。7%的记录C6胶质瘤细胞未显示任何外向钾电流。其余细胞具有外向K+电流，可在从-70 mV的钳制电位激活至-36.5±5.9 mV时出现。尾电流分析显示，反转电位的平均值为-84.6±4.1 mV，接近K+平衡电位。60%记录的C6细胞的K+电流由快速和慢速成分组成，而33%的细胞仅具有慢速成分。
