Xie Z, He S F
Shanghai Brain Research Institute, Chinese Academy of Sciences.
Sheng Li Xue Bao. 1997 Feb;49(1):45-53.
In this study, the inhibitory effects of R(-)-NPA (R(-)-Propylnorapomorphine) and haloperidol on voltage-dependent K+ currents in rat C6 glioma cells (C6 cells) were observed by means of the whole-cell patch-clamp recording technique. The mechanisms of the effects were also analyzed. The main results were as follows: (1) Both R(-)-NPA and haloperidol could inhibit K+ currents dose-dependently and reversibly in C6 cells. (2) R(-)-NPA and haloperidol inhibited mainly the slow component of the K+ currents. (3) These inhibitory responses were not mediated by dopamine receptors or dependent on G-proteins and intracellular calcium. It was suggested that they refer to some direct action on K+ channels.
在本研究中，采用全细胞膜片钳记录技术观察了R(-)-NPA（R(-)-丙基去甲阿扑吗啡）和氟哌啶醇对大鼠C6胶质瘤细胞（C6细胞）电压依赖性钾电流的抑制作用，并对其作用机制进行了分析。主要结果如下：(1) R(-)-NPA和氟哌啶醇均可剂量依赖性且可逆地抑制C6细胞的钾电流。(2) R(-)-NPA和氟哌啶醇主要抑制钾电流的慢成分。(3) 这些抑制反应不是由多巴胺受体介导的，也不依赖于G蛋白和细胞内钙。提示它们对钾通道有一些直接作用。
