Gao T L, Huang Y Z
College of Life Sciences, Peking University, Beijing.
Sheng Li Xue Bao. 1997 Apr;49(2):178-84.
The protective effects of ischemic preconditioning on ischemia-reperfusion injury was investigated using isolated Langendorff perfusing hearts from ground squirrel and rat. In Preconditioning I group hearts were first perfused with Krebs-Henseleit solution for 10 min to establish a steady state, then stopped for 15 min to establish global ischemia, and finally followed by 10 min ischemia and 10 min reperfusion. In Preconditioning II group there were three cycles of 5 min ischemia + 5 min reperfusion after 10 min equilibration and then the final 10 min ischemia and 10 min reperfusion were followed. It was found that in group I during the final 10 min ischemia period there was remarkable augmentation of CK release from both animal's hearts. But in group II CK release decreased markedly during the same ischemic period. CK release during final 10 min reperfusion period also decreased significantly in group II in comparison with group I. The incidence of arrhythmias occurred in both animal's hearts was markedly reduced in group II rather than group I. In conclusion, short episode ischemic preconditioning protect subsequent ischemia-reperfusion injury on isolated hearts from ground squirrel and rat.
采用地松鼠和大鼠的离体Langendorff灌注心脏，研究缺血预处理对缺血再灌注损伤的保护作用。在预处理I组中，心脏首先用Krebs-Henseleit溶液灌注10分钟以建立稳定状态，然后停灌15分钟以建立全心缺血，最后进行10分钟缺血和10分钟再灌注。在预处理II组中，平衡10分钟后进行三个5分钟缺血+5分钟再灌注的循环，然后进行最后的10分钟缺血和10分钟再灌注。结果发现，在I组中，在最后的10分钟缺血期，两种动物心脏的CK释放均显著增加。但在II组中，在相同的缺血期CK释放明显减少。与I组相比，II组在最后的10分钟再灌注期CK释放也显著降低。II组两种动物心脏发生心律失常的发生率明显低于I组。总之，短时间的缺血预处理可保护地松鼠和大鼠离体心脏免受随后的缺血再灌注损伤。
