降钙素基因相关肽介导的离体大鼠心脏缺血预处理
Ischemic preconditioning mediated by calcitonin gene-related peptide in isolated rat hearts.
作者信息
Xiao Z S, Li Y J, Deng H W
机构信息
Department of Pharmacology, Hu-nan Medical University, Changsha, China.
出版信息
Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):445-8.
AIM
To study the mediating effect of calcitonin gene-related peptide (CGRP) in ischemic preconditioning in the isolated perfused rat heart.
METHODS
Isolated rat hearts were subjected to 3 cycles of a 5-min ischemia and a 5-min reperfusion before a 30-min global ischemia followed by a 30-min reperfusion.
RESULTS
Ischemic preconditioning caused an improvement of heart functions, reduced the incidence of ventricular arrhythmias and decreased the release of creatine kinase (CK) during reperfusion (CK activities = 0.30 +/- 0.07, 2.03 +/- 0.49, and 0.92 +/- 0.40 U.min-1/g wet wt for control, ischemia-reperfusion, and preconditioning, respectively). However, pretreatment with CGRP8-37 (0.1 mumol.L-1) abolished the improvement of cardiac contractivity, the reduction of the incidence of arrhythmias, and the inhibition of CK release by preconditioning (CK activities = 0.9 +/- 0.4 vs 2.55 +/- 0.32 U.min-1/g wet wt, P < 0.01).
CONCLUSION
CGRP is an endogenous myocardial protective substance that played an important role in mediation of ischemic preconditioning.
目的
研究降钙素基因相关肽（CGRP）在大鼠离体灌注心脏缺血预处理中的介导作用。
方法
离体大鼠心脏在30分钟全心缺血及随后30分钟再灌注之前，先经历3个5分钟缺血和5分钟再灌注的循环。
结果
缺血预处理可改善心脏功能，降低室性心律失常的发生率，并减少再灌注期间肌酸激酶（CK）的释放（对照组、缺血-再灌注组和预处理组的CK活性分别为0.30±0.07、2.03±0.49和0.92±0.40 U·min⁻¹/g湿重）。然而，用CGRP8-37（0.1 μmol·L⁻¹）预处理可消除预处理对心脏收缩性的改善、心律失常发生率的降低以及CK释放的抑制作用（CK活性=0.9±0.4对2.55±0.32 U·min⁻¹/g湿重，P<0.01）。
结论
CGRP是一种内源性心肌保护物质，在缺血预处理的介导中起重要作用。
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