Liu Q S, Jia Y S, Xie Z P, Ju G
Institute of Neuroscience, The Fourth Military Medical University, Xi'an.
Sheng Li Xue Bao. 1997 Aug;49(4):467-70.
Fifty-two supraoptic nucleus neurons in rat slice preparations were studied using whole cell patch-clamp technique. The mean passive and active membrane properties were measured as follows: resting membrane potential, 59 +/- 8 mV; input resistance, 535 +/- 129 M omega; time constant, 32 +/- 9 ms; amplitude of the action potentials, 99 +/- 11 mV; overshoot, 37 +/- 13 mV (n = 39). Most of these neurons showed a prominent slow after-hyperpolarization potential or current in response to depolarizing pulse. In votage-clamp condition, it was found that virtually all supraoptic neurons (n = 13) were invaded by spontaneous synaptic inputs. Pharmacological experiments showed that the excitatory postsynaptic currents (EPSCs) were mediated by non-NMDA glutamate receptors, whereas inhibitory postsynaptic currents (IPSCs) by GABAA receptors.
使用全细胞膜片钳技术对大鼠脑片制备中的52个视上核神经元进行了研究。测量的平均被动和主动膜特性如下：静息膜电位，59±8 mV；输入电阻，535±129 MΩ；时间常数，32±9 ms；动作电位幅度，99±11 mV；超射，37±13 mV（n = 39）。这些神经元中的大多数在去极化脉冲刺激下表现出明显的慢后超极化电位或电流。在电压钳条件下，发现几乎所有视上核神经元（n = 13）都受到自发突触输入的侵袭。药理学实验表明，兴奋性突触后电流（EPSCs）由非NMDA谷氨酸受体介导，而抑制性突触后电流（IPSCs）由GABAA受体介导。
