[Alteration of sensitivity of supraoptic nucleus neurons to cytokine in the hypothalamic slices from the rat after lipopolysaccharide injection].

Using the whole cell patch clamp technique, the effects of interleukin 1beta (IL-1beta) and IL-2 on the membrane potential and spontaneous discharges in the supraoptic nucleus (SON) neurons from the rat hypothalamic slices in vitro were examined to determine the changes in sensitivity of supraoptic nucleus neurons to cytokine. The results obtained are as follows. IL-1beta (100 U/ml) hyperpolarized the SON neurons from the control rats (n=15) and the rats 9 d after i.p. lipopolysaccharide (LPS) (n=20), and decreased the rate of their spontaneous discharges. When 100 U/ml IL-2 was perfused, most SON neurons (n=14) from the control rats were hyperpolarized and the spontaneous firing decreased, but the rest (n=3) remained unchanged. When IL-2 (100 U/ml) was applied, 19 neurons out of the 45 SON neurons from the rats 9 days after i.p. LPS were depolarized accompanied with an increase of the discharges, 16 did not show significant changes in both membrane potential and spontaneous discharges, the rest 10 were hyperpolarized and their discharges were suppressed. These results show that the sensitivity of part of SON neurons from the immuned rat to cytokine IL-2 was altered, suggesting that IL-2 may be involved in modulation of the activity of SON neurons and thus play a role in modulation of the immune response.